After picking up a groin injury in a friendly this week, Omid Ebrahimi was left off coach Carlos Queiroz's 25-man list on Sunday.
Azmoun, 27, tore his right calf muscle while warming up for Bayer Leverkusen's Champions League clash with Porto on Oct. 4, and has not played since.
In Thursday's friendly against Nicaragua, Al Wakrah midfielder Ebrahimi suffered a groin injury. Since November, he has only made four appearances for Iran under Queiroz.
When Mehdi Taremi returns from injury in time for the Nov. 21 match against England, he will lead the line alone or in tandem with Azmoun.
Tunisia will be Iran's final warm-up opponent before taking on England, Wales and United States in Group B.
Goalkeepers: Alireza Beiranvand (Persepolis), Amir Abedzadeh (Ponferradina), Hossein Hosseini (Esteghlal), Payam Niazmand (Sepahan).
Defenders: Ehsan Hajsafi (AEK Athens), Morteza Pouraliganji (Persepolis), Ramin Rezaeian (Sepahan), Milad Mohammadi (AEK Athens), Hossein Kanaanizadegan (Al Ahli), Shoja' Khalilzadeh (Al Ahli), Sadegh Moharrami (Dinamo Zagreb), Rouzbeh Cheshmi (Esteghlal), Majid Hosseini (Kayserispor), Abolfazl Jalali (Esteghlal).
Midfielders: Ahmad Nourollahi (Shabab Al Ahli), Saman Ghoddos (Brentford), Vahid Amiri (Persepolis), Saeid Ezatolahi (Vejle), Alireza Jahanbakhsh (Feyenoord), Mehdi Torabi (Persepolis), Ali Gholizadeh (Charleroi), Ali Karimi (Kayserispor).
Forwards: Karim Ansarifard (Omonia Nicosia), Sardar Azmoun (Bayer Leverkusen), Mehdi Taremi (Porto).